Fig. 2.

Effect of plate conservation 24 h at 4 °C (a), 24 h at room temperature (b) and impact of the absence of BSA carrier for peptides conjugation to beads (c). This figure shows the effect of delayed acquisition of MFI signal after 24 h at 4 °C (a) and 24 h at room temperature (b). Individual antibody responses against the different antigens are shown as dot blot and arrow to the corresponding delayed measure. A red arrow links particular individual measures that did not decrease. The decrease of level was significant (P < 10⁻³, Wilcoxon signed rank test for paired data) ranging from 5% to 16% after 24 h at 4 °C, and from 17% to 25% after 24 h at RT. c shows the comparison of antibody responses against CSP and PfAMA1 peptides with (black) or without BSA (light grey); levels of responses are significantly lower without BSA (P < 10⁻³, Wilcoxon signed rank test)