Table 1.
EC50s of WT and mutant GlyR
| Mutant | pEC50 (M) | EC50 (μM) | Mutant | pEC50 (M) | EC50 (μM) |
|---|---|---|---|---|---|
| WT | 4.380 ± 0.04 | 42 | F293A | 3.559 ± 0.06* | 276 |
| F293Y | 3.901 ± 0.02* | 125 | |||
| M1 | F295A | 4.339 ± 0.07 | 46 | ||
| Y222A | NR | Y301A | 3.463 ± 0.09* | 345 | |
| Y222F | 3.519 ± 0.03* | 303 | F306A | 3.795 ± 0.03* | 160 |
| Y223A | NR | ||||
| Y223F | 3.721 ± 0.07* | 190 | M4 | ||
| Y228A | NR | F395A | 3.724 ± 0.05* | 189 | |
| Y228F | 4.466 ± 0.07 | 34 | F399A | NR | |
| W239A | NR | F399Y | 3.846 ± 0.04* | 143 | |
| W239F | 4.248 ± 0.04 | 57 | F402A | 4.183 ± 0.03 | 66 |
| F242A | 3.803 ± 0.07* | 157 | F402Y | 3.725 ± 0.01* | 188 |
| W243A | 3.764 ± 0.06* | 172 | F405A | 2.961 ± 0.07* | 1095 |
| F405Y | 3.718 ± 0.07* | 192 | |||
| M3 | Y406A | NR | |||
| W286A | NR | W407A | 3.366 ± 0.04* | 430 | |
| W286Y | 2.351 ± 0.05* | 445 | Y410A | 3.489 ± 0.16* | 324 |
Data = mean ± SEM, n = 3–8; * = significantly different to WT, p < 0.05 ANOVA with Dunnetts multiple comparison post-test; NR = non-responsive