Fig. 7.

Down-regulation of GhSIF1 leads to enhanced salt tolerance in VIGS treated cotton plants. Ten days old cotton plants (G. hirsutum) with two cotyledon leaves were infiltrated with TRV1 and empty TRV2 (as control) or TRV2-GhSIF1 (targeting GhSIF1 mRNA). Ten days later, plants were treated with 300 mM NaCl for 2 weeks. a Pictures were taken 18 days after salt treatment. b Shoot length and Root length, c fresh weight, d dry weight, e chlorophyll content, and f electrolyte leakage of control plants and GhSIF1 targeting plants were measured. For (B-D) data shown are an average of eight independent biological replicates. Error bars represent S.D. (n = 8). P < 0.05 was marked as *. P < 0.01 was marked as **. For (E-F) data shown are an average of three technical replicates for five independent biological replicates. Error bars represent S.D. (n = 15). P < 0.05 was marked as *. P < 0.01 was marked as **. VIGS(empty): control plant. VIGS(GhSIF1): GhSIF1 targeting plant