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. 2018 Aug 28;7:e37082. doi: 10.7554/eLife.37082

Figure 5. AMB induces changes in RGL2 and ABI5 accumulation in a manner similar to that observed with P. aeruginosa or WT PAO1 extracts.

Figure 5.

(A) Protein gel blot analysis of a time course of RGL2 and ABI5 protein levels upon seed imbibition in the absence (MS) or presence of 50 µM AMB (AMB). UGPase protein levels are used as a loading control. Top panel: RGL2 and ABI5 protein levels in WT seeds. Bottom panel: ABI5 protein levels in Δdella seeds. Germination percentage at each time point is indicated. The asterisk (*) represents and unspecific banc detected by the RGL2 antibody. (B) Same as in A using seeds imbibed in the presence of 10 µM PAC, 1 µM GA or 100 µM AMB as indicated. Germination percentage at each time point is indicated.