Skip to main content
. Author manuscript; available in PMC: 2018 Sep 7.
Published in final edited form as: Processes (Basel). 2018 Apr 25;6(5):42. doi: 10.3390/pr6050042

Figure 6.

Figure 6.

Depletion of DFFA protein expression and the effect of miR-140–3p on cellular growth. (A) Representative blots for DFFA and β-actin (loading control) are shown (n = 3). The miRNA is able to bind and regulate the DFFA gene in MCF-7, but not in MDA-MB-231 due the presence of an A-to-I edit. WT, wild type; Ctl, empty lipo transfection; Ant-140, miR-140 antagomir; Ant-Ctl, random antagomir. (B) Cell growth assay examining effects of transfecting a miR-140 inhibitor in both cell lines. Five microscopic fields randomly chosen from each assay were counted individually, and the statistical significance between treatment and control determined by t-test.