Table 1.
List of top 10 miRs where ADAR editing of mRNAs alters complementarity to miR seed regions and either (A) creates novel target sites for regulation or (B) destroys predicted target sites. In addition to altered edit complementarity, microRNAs included were also required to be present at >50 reads per million in MCF-7 and MDA-MB-231 small RNA-Seq datasets.
| miR | miRBase ID | Seed (RC) | Targets (Edited) |
Targets (Unedited) |
Expected |
|---|---|---|---|---|---|
| A | |||||
| hsa-miR-513a-5p | MIMAT0002877 | CCTGTGA | 258 | 0 | 0.63 |
| hsa-miR-450b-3p | MIMAT0004910 | GATCCCA | 252 | 4 | 0.79 |
| hsa-miR-769–3p | MIMAT0003887 | GATCCCA | 252 | 4 | 0.79 |
| hsa-miR-6089 | MIMAT0023714 | CGGCCTC | 219 | 0 | 3.83 |
| hsa-miR-4691–3p | MIMAT0019782 | GTGGCTG | 181 | 0 | 1.16 |
| hsa-miR-3189–3p | MIMAT0015071 | CCCAAGG | 140 | 5 | 0.48 |
| hsa-miR-140–3p | MIMAT0004597 | CTGTGGT | 139 | 0 | 1.11 |
| hsa-miR-3065–3p | MIMAT0015378 | GGTGCTG | 118 | 0 | 0.5 |
| hsa-miR-3940–3p | MIMAT0018356 | CCGGGCT | 111 | 0 | 0.72 |
| hsa-miR-3680–3p | MIMAT0018107 | ATGCAAA | 108 | 2 | 0.82 |
| B | |||||
| hsa-miR-5089–5p | MIMAT0021081 | AATCCCA | 0 | 644 | 21.39 |
| hsa-miR-6504–3p | MIMAT0025465 | CTGTAAT | 58 | 587 | 19.93 |
| hsa-miR-6506–5p | MIMAT0025468 | ATCCCAG | 18 | 377 | 21.57 |
| hsa-miR-619–5p | MIMAT0026622 | ATCCCAG | 18 | 377 | 21.57 |
| hsa-miR-4775 | MIMAT0019931 | AAAATTA | 0 | 351 | 19.37 |
| hsa-miR-4735–5p | MIMAT0019860 | AAATTAG | 6 | 305 | 17.31 |
| hsa-miR-6514–3p | MIMAT0025485 | ACAGGCA | 10 | 216 | 9.59 |
| hsa-miR-4794 | MIMAT0019967 | TAGCCAG | 10 | 173 | 8.05 |
| hsa-miR-664a-5p | MIMAT0005948 | TAGCCAG | 10 | 173 | 8.05 |
| hsa-miR-1273e | MIMAT0018079 | TCAAGCA | 2 | 169 | 5.22 |