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. 2018 Aug 2;10(8):1932–1946. doi: 10.18632/aging.101513

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Copyright © 2018 Hu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Pathological changes of the cornea of Sirt6 KO mice after injury. 2.0 mm central corneal epithelial debridement wounds were induced in 8-week-old WT and Sirt6 KO mice and eyes were collected 3 weeks after injury. (A) Histological appearance of corneas with H&E staining at 200X magnification (upper panel) and 400X magnification (lower panel). (B) Immunostaining of corneal epithelium marker keratin 12, and the keratinization-related protein loricrin (red) in corneal cryosections from WT and Sirt6 KO mice. Blue: DAPI staining for nuclei. Scale bar: 50 μm.