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. 2018 Aug 2;10(8):1932–1946. doi: 10.18632/aging.101513

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Copyright © 2018 Hu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Sirt6^-/- mice develop corneal plaques with infiltration of inflammatory cells and neovascularization as they age. Eyes were collected from 7-month-old Sirt6 KO mice and their littermate WT mice. (A) Images of the corneas and H&E staining of cross-sections of eyes from WT and Sirt6 KO mice (n=6 mice). Scale bar: 50 μm. (B) Immunostaining of keratin 12 (red) and loricin (green). Scale bar: 100 μm. (C) Immunostaining for hematopoietic cell markers such as CD45 for lymphoid cells (monocytes) (green), Iba1 for activated macrophages, MPO for neutrophils, CD3 for T cells, CD19 for B cells and CD31 for neovascularization in 7 month-old WT and Sirt6 KO corneas. Nuclei are counterstained with DAPI (blue). Scale bar: 100 μm.