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. 2018 Aug 27;10(8):2098–2112. doi: 10.18632/aging.101532

Figure 5.

Figure 5

Tunicamycin treatment induces ER stress but not autophagy in lungs from Atg4b-/- mice. (A) Representative immunoblots of ER stress biomarkers in lung tissue from WT vehicle-control and WT tunicamycin-treated mice at 3 days post-challenge. (B) Representative immunoblots of ER stress biomarkers in lung tissue from Atg4b vehicle-control and Atg4b tunicamycin-treated null mice at 3 days post-challenge. (C) Representative immunoblots of ATG4B, LC3-I/ II, and p62 in lung tissue from WT vehicle-control and WT tunicamycin-treated mice at 3 days post-challenge. (D) Representative immunoblots of ATG4B, LC3-I/ II, and p62 in lung tissue from WT compared to Atg4b null mice after 3 days of tunicamycin treatment. β-tubulin was used as loading control in all experiments. Densitometry analysis (bottom panels). Results are shown as mean ± SD. Statistical significance was determined by Student´s t-test (*p < 0.05).