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. 2018 Aug 9;10(8):1884–1901. doi: 10.18632/aging.101510

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Copyright © 2018 Lian et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY) 3.0 License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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ANLN is highly expressed in hepatocellular carcinoma. (A) The mRNA levels of ANLN in global human cancer tissues (red) and non-tumor tissues (blue) were analyzed using the TCGA database (http://firebrowse.org/). Noticeably, the fold change of tumor vs normal tissue in hepatocellular carcinoma was 16.3. ESCA: Esophageal carcinoma; HNSC: Head and neck squamous cell carcinoma; CESC: Cervical squamous cell carcinoma and endocervical adenocarcinoma; STES: Stomach and esophageal carcinoma; LUSC: Lung squamous cell carcinoma; SARC: Sarcoma; STAD: Stomach adenocarcinoma; COAD: Colon adenocarcinoma; COADREAD: Colon and rectum adenocarcinoma; BLCA: Bladder urothelial carcinoma; READ: Rectum adenocarcinoma; SKCM: Skin Cutaneous melanoma; GBMLGG: Glioblastoma multiforme and brain lower grade glioma (GBM + LGG); GBM: Glioblastoma multiforme; LUAD: Lung adenocarcinoma; BRCA: Breast invasive carcinoma; UCEC: Uterine corpus endometrial carcinoma; PAAD: Pancreatic adenocarcinoma; CHOL: Cholangiocarcinoma; THYM: Tymoma; LIHC: Liver hepatocellular carcinoma; KIRC: Kidney renal clear cell carcinoma; KICH: Kidney chromophobe; KIRP: Kidney renal; KIPAN: Pan-kidney cohort (KICH + KIRC+ KIRP); PCPG: Pheochromocytoma and paraganglioma; PRAD: Prostate adenocarcinoma; THCA: Tyroid carcinoma. (B) Representative image of ANLN expression in HCC tissue and matched adjacent tissue by IHC and HE analyses. Scale bar for the left panel: 500 μm; Scale bar for the right panel: 50 μm. T: tumor; N: non-tumor. (C) Quantification of the percentage of ANLN-positive cells in tumor tissues and non-tumor tissues from 201 HCC slices. ***P < 0.001. (D) The mRNA levels of ANLN from 81 pairs of HCC tissues and matched adjacent non-tumor tissues were tested by quantitative PCR. GAPDH was used as an internal control. ***P < 0.001. (E) The protein levels of ANLN in 9 pairs of HCC tissues and matched non-tumor tissues were determined by western blotting assay. The relative fold changes of T compared with N are shown below. T: tumor; N: non-tumor. (F) ANLN expression in HCC cell lines. The relative fold changes of all cell lines compared with MIHA are shown below.