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. 2018 Sep 7;13(9):e0203402. doi: 10.1371/journal.pone.0203402

Fig 5. Synergistic effects of MEK and JNK inhibition with cetuximab shows that different signalling pathways act simultaneously.

Fig 5

The HPV- HNC cell line UM-SCC4 and the HPV+ HNC cell line UM-SCC47 were stimulated with 1μg/mL rituximab or 1μg/mL cetuximab as indicated for 72h, a specific inhibitor as indicated for 48h, 50IU/mL IFNγ and 30ng/ml TNFα as indicated for 24h. The expression levels of the genes indicated were determined by RT-qPCR. Gene expression was normalized against GAPDH mRNA levels. (A)50μM of PD98059 (MEK1 inhibitor) was used. (B)20μM of SP600125 (JNK inhibitor) was used. Similar results were observed in two independent experiments. P value were determined by unpaired t-tests. Ns: no significance. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.