Table 3. Minimal inhibitory concentrations (MICs) and minimal bactericidal concentrations (MBCs) against microorganisms and hemolysis activity percentage of peptides.
| AMP | MIC (μM) | MBC (μM)1 | Hemolysis activity (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| X.ciri | S. meliloti | Met sp. | A. t | X.ciri | S. meliloti | Met sp. | A.t | 25 mM | 50 mM | 75 mM | 100 mM | |
| Citrus-amp1 | 32 | 64 | 64 | 64 | 32 | 64 | 64 | ND* | 1 | 0 | 0 | 1 |
| Citrus-amp2 | ND | 64 | ND | ND | ND | 64 | ND | ND | 1 | 0 | 0 | 0 |
| K0-W6-Hy-a1 | 2 | 4 | 4 | ND | 2 | 62 | 8 | ND* | 14 | 53 | 83 | 99 |
| Ocellatin4-Analogue1 | 3 | 3 | 3 | 3 | 3 | 14 | 7 | 55* | 88 | 100 | 100 | 100 |
| Tritrpticin | 8 | 4 | ND | ND | 8 | 8 | ND | ND | 1 | 3 | 10 | 28 |
| Oxytocin2 | - | - | - | - | - | - | - | - | 0 | 0 | 0 | 1 |
| PBS3 | - | - | - | - | - | - | - | - | - | - | - | - |
| Hylin-a14 | 4 | ND | 9 | 17 | 9 | ND | 17 | 17 | - | - | - | - |
X.citri–Xanthomonas citri subsp. citri; S.meliloti–Sinorhizobium meliloti; Met sp.–Methylobacterium sp.; A.t–Agrobacterium tumefaciens; ND: Not determined (higher than the concentrations evaluated).
*Growth reduction
1Positive control for hemolysis
2Negative control for hemolysis activity
3Negative control for MIC/MBC
4 Positive control for MIC/MBC.