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. 2018 Sep 7;13(9):e0203517. doi: 10.1371/journal.pone.0203517

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© 2018 Reese et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Calculated mean percent growth inhibition of increasing concentrations of chlorambucil (0.01 to 1000 μM) against in vitro SF767 (gray dotted line) and U87-MG (black solid line) glioma cell lines as determined using the Sulforhodamine B assay. Data are expressed in the figure by the % of growth retained under each condition, compared to the control. The IC₅₀ was then determined to be 114 μM and 96 μM for the SF767 and U87-MG glioma cell lines, respectively.