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. 2018 Sep 7;13(9):e0203608. doi: 10.1371/journal.pone.0203608

Fig 3. RNA integrity from rat PET blocks decrease depending on storage temperature.

Fig 3

For each of five different tissue types (liver, kidney, spleen, lung and intestine) and four different temperatures (22°C, 4°C, -20°C, and -80°C) 3 blocks of FFPE and PFPE rat tissue were stored. RNA was extracted from block 1 after 3, 6 and 12 months (m), from block 2 after 24, 36, 48 and 72 m and from block 3 after 108 m of storage. Mean RNA integrity number (RIN) values with standard deviation from FFPE (A) and PFPE tissue (B) are shown for triplicate extractions from five different tissue types for each fixation method, storage time point and temperature (total RIN values n = 942). Dotted line indicates block change.