Table 2. Antibodies used in western blots.

Primary antibody	Antigen specificity or immunogen	Secondary antibody	Company/Catalog #
Anti-fluorescein	KLH-bound fluorescein	M	Sigma Aldrich/11426320001
Neprilysin (anti-CD10)	Synthetic peptide corresponding to Human CD10 aa 50–500	R	Abcam/ab126593
Cathepsin D	Human cathepsin D	M	BD Transduction Labs/610800
IDE	purified human erythrocyte IDE	M	Covance/mms-282R
GAPDH	Full-length human GAPDH protein	M	Life Technologies/39-8600

aa, amino acid; CTF, C-terminal fragment; IDE, insulin degrading enzyme; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; M, HRP conjugated AffiniPure goat-anti mouse IgG (catalog # 115-035-146, Jackson Laboratory); R, HRP conjugated AffiniPure goat-anti rabbit IgG, (catalog # 111-035-144, Jackson Laboratory).