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. 2018 Sep 7;13(9):e0203659. doi: 10.1371/journal.pone.0203659

Table 2. Antibodies used in western blots.

Primary antibody Antigen specificity or immunogen Secondary antibody Company/Catalog #
Anti-fluorescein KLH-bound fluorescein M Sigma Aldrich/11426320001
Neprilysin (anti-CD10) Synthetic peptide corresponding to Human CD10 aa 50–500 R Abcam/ab126593
Cathepsin D Human cathepsin D M BD Transduction Labs/610800
IDE purified human erythrocyte IDE M Covance/mms-282R
GAPDH Full-length human GAPDH protein M Life Technologies/39-8600

aa, amino acid; CTF, C-terminal fragment; IDE, insulin degrading enzyme; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; M, HRP conjugated AffiniPure goat-anti mouse IgG (catalog # 115-035-146, Jackson Laboratory); R, HRP conjugated AffiniPure goat-anti rabbit IgG, (catalog # 111-035-144, Jackson Laboratory).