Fig 4. FAs from lipid droplets sustain activated phospholipid synthesis in infected cells.

A. Incorporation of a choline analog propargylcholine into the membranes of infected cells. HeLa cells were infected with poliovirus at an MOI of 10 PFU/cell, and were incubated with the indicated inhibitors after infection in balanced Earle solution. Orlistat is an inhibitor of de novo FA synthesis by FASN, DEUP is an inhibitor of mobilization of neutral lipids stored in lipid droplets. At 5 h p.i., the incubation medium was replaced with fresh pre-warmed balanced Earle solution containing propargylcholine. The cells were fixed at 6 h p.i. and processed for click-chemistry-based detection of incorporated propargylcholine. B. Quantitation of propargylcholine incorporation by HeLa cells infected and processed as in A.