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The adhesive forces were calculated by averaging 30 measurements on three individual surfaces. The forces for control, after 10 puffs and 150 puffs were (1.2 ± 0.4, 2.2 ± 0.5, and 4.5 ± 2.6) nN, respectively (mean ± S.D.). Student t-tests were performed control vs. individual puffing regime (*** = p<0.0001).
