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. 2018 Aug 30;13(7):e1486146. doi: 10.1080/15592324.2018.1486146

Figure 1.

Figure 1.

Phenotype of mutants impaired in the expression of bHLH transcription factors. A Intron-exon structure of the bHLH093 and bHLH061 genes in the Arabidopsis genome and location of T-DNA insertion sites. The box with dotted line indicates the promoter region. B RT-PCR with primers designed to amplify the bHLH093 and bHLH061 genes (note the absence of transcript for bHLH093 in the bhlh093-1 mutant, for bHLH061 in the bhlh061-1 mutant, and for both bHLH093 and bHLH061 in the bhlh093-1/bhlh061-1 double mutant). C Delayed bolting phenotype in bhlh mutants (size bar 1 cm). D Biometric evaluation of bhlh093-1/bhlh061-1 double mutant (striped bars) and wild-type controls (hollow bars) (standard deviation is indicated). Asterisks indicate P-value < 0.01 in a two-way ANOVA. There is no difference in the stomatal density on leaves of wild-type control (E) and the bhlh093-1/bhlh061-1 double mutant (F). In the lower panels, black dots are added to indicate the position of stomata. G Transformation of the bhlh093-1/bhlh061-1 double mutant with a functional copy of bHLH093 partially complements the bolting phenotype, while bHLH093 overexpressors do not have an obvious phenotype (size bar 1 cm).