Key resources table.
| Reagent type | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (human) |
HeLa parental | Provided by Y. Mimori-Kiyosue |
||
| Cell line (human) |
GFP-Aurora B HeLa |
Provided by M. Lampson |
||
| Cell line (human) |
Cyclin B1- Venus HeLa |
Provided by J. Pines |
||
| Antibody | anti-Aurora B pT232 (Rabbit Polyclonal) |
Rockland Immuno chemicals |
Cat#600-401-677 | IF: (1:500) |
| Antibody | anti-Aim1 (Mouse Monoclonal) |
BD Biosciences | Cat#611083 | IF: (1:500) WB: (1:1,000) |
| Antibody | anti-Mad1 (Mouse Monoclonal) |
Merck Millipore | Cat#MABE867 | IF: (1:500) |
| Antibody | anti-phospho- Histone H3 Ser10 (Mouse Monoclonal) |
Abcam | Cat#ab14955 | IF: (1:100,000) |
| Antibody | anti-Cleaved Caspase-3 (Rabbit Polyclonal) |
Cell Signaling Technology |
Cat#9661 | IF: (1:1,000) |
| Antibody | anti-Centromere antibody (Human) |
Fitzgerald | Cat#90C-CS1058 | IF: (1:500) |
| Antibody | anti-phospho- Mps1 Thr676 (Rabbit Polyclonal) |
a gift from G. Kops | IF: (1:2,000) | |
| Antibody | anti-phospho- Histone H3 Thr3 (Mouse Monoclonal) |
a gift from J. Higgins |
IF: (1:1,000) | |
| Antibody | anti-HEC1 (Mouse Monoclonal) |
a gift from R. Gassmann |
IF: (1:2,000) | |
| Antibody | anti-KNL1 (Rabbit Polyclonal) |
a gift from R. Gassmann |
IF: (1:500) | |
| Antibody | anti-phosho DSN1 (Rabbit Polyclonal) |
a gift from I. Cheeseman |
IF: (1:1,000) | |
| Antibody | anti-phospho KNL1 (Rabbit Polyclonal) |
a gift from I. Cheeseman |
IF: (1:1,000) | |
| Antibody | anti-CENPA (Mouse Monoclonal) |
a gift from L. Jansen |
IF: (1:200) | |
| Antibody | anti-DSN1 (Mouse Monoclonal) |
a gift from A. Musacchio |
IF: (1:200) | |
| Antibody | Alexa 488- or 568-or 647 secondaries |
Invitrogen | IF: (1:1,000) | |
| Antibody | anti-Survivin (Rabbit Polyclonal) |
Novus Biologicals |
Cat#NB500-201 | WB: (1:1,000) |
| Antibody | anti-Borealin (Rabbit Polyclonal) |
a gift from R. Gassmann |
WB: (1:1,000) | |
| Antibody | anti-α-tubulin clone B-512 (Mouse Monoclonal) |
Sigma-Aldrich | Cat#T5168 | WB: (1:5,000) |
| Antibody | anti-RNA Pol II S2 (Rabbit Polyclonal) |
Abcam | Cat#ab5095 | WB: (1:1,000) |
| Antibody | anti-mouse or anti-rabbit |
Jackson Immuno Research |
WB: (1:5,000) | |
| Chemical compound, drug |
Nocodazole | Sigma-Aldrich | Cat#M1404 | 1 μM |
| Chemical compound, drug |
MG132 | EMD Millipore | Cat#133407-82-6 | 5 μM |
| Chemical compound, drug |
Mps1-IN-1 | Provided by N. Gray |
10 μM | |
| Chemical compound, drug |
RO3306 | Roche | Cat#SML0569 | 10 μM |
| Chemical compound, drug |
Actinomycin D | Sigma-Aldrich | Cat#A9415 | 8 μM |
| Chemical compound, drug |
α-amanitin | Sigma-Aldrich | Cat#A2263 | 2 μM |
| Chemical compound, drug |
Triptolide | Sigma-Aldrich | Cat#T3652 | 1 μM |
| Chemical compound, drug |
Cycloheximide | Sigma-Aldrich | Cat#01810 | 35.5 μM |
| Chemical compound, drug |
Ethidium Bromide |
Sigma-Aldrich | Cat#E8751 | 25 μM |
| Chemical compound, drug |
ZM447439 (Aurora B inhibitor) |
Selleckchem.com | Cat#S1103 | 3.3 μM |