Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | anti-HA | Santa Cruz | SC-7392 | |
| Antibody | anti-HA beads | Sigma | A2095 | |
| Antibody | anti-Flag | Sigma | F3165 | |
| Antibody | anti-Flag beads | Sigma | A2220 | |
| Antibody | anti-Myc | This study | ||
| Antibody | anti-G6PDH | Sigma | A9521 | |
| Antibody | anti-Smt3 (SUMO) | Santa Cruz | SC-28649 | |
| Antibody | GFP-Trap beads | chromotek | gta-20 | |
| Antibody | Alexa Fluor 680 Goat anti-Mouse IgG |
Fisher Scientific | A21058 | |
| Antibody | Alexa Fluor 680 Goat anti-Rabbit IgG |
Fisher Scientific | A21109 | |
| Antibody | DyLight 800 Goat anti-Rabbit IgG |
Fisher Scientific | SA535571 | |
| Antibody | DyLight 800 Goat anti-Mouse IgG |
Fisher Scientific | SA535521 | |
| Peptide, recombinant protein |
2x HA peptide | This study | ||
| Peptide, recombinant protein |
2x Flag peptide | This study | ||
| Commercial assay or kit |
cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail |
Sigma | 11836170001 | |
| Commercial assay or kit |
Power SYBR Green PCR Master Mix |
Fisher Scientific | 4367659 | |
| Commercial assay or kit |
SuperScript III First-Strand Synthesis System |
Invitrogen | 18080–051 | |
| Chemical compound, drug |
N-Ethylmaleimide (NEM) | Sigma | E3876 | |
| Chemical compound, drug |
5-Fluoroorotic Acid (5-FOA) |
Toronto Research Chemicals |
F595000 | |
| Chemical compound, drug |
MG132 | Fisher Scientific | 50-833-9 | |
| Chemical compound, drug |
Cycloheximide | Sigma | C7698 | |
| Software, algorithm |
Excel | Microsoft |