Figure 3.

Disruption of the N-linked glycosylation sequence in rho^fl10 and rho^fl7 leads to rod degeneration. A, C: Chromatograms overlaid with amino acid sequences comparing WT, rho^fl10, and rho^fl7 alleles, disrupting the conserved NXT consensus glycosylation sequence at N15. Red highlights deleted amino acids, while blue highlights insertions. B, D: Confocal images of serial retinal cryosections of 5 or 6 dpf WT or heterozygous rho^fl10/+ and rho^fl7/+ mutants labeled with antibodies to Rho (1D1, red), rods (4C12, red), and Arr3a (Zpr-1, red) overlaid with bright-field microscopy reveal loss of rod-specific labeling in the central retina. E: Higher magnification images of the dorsal retinal margin from WT, heterozygous rho^fl10/+, or rho^fl7/+ mutants showing sparse immunolabeling for Rho and rods.