Table 1. Frequency of and rhodopsin mutations recovered in F1 following gene targeting in zebrafish.

Target	Transmitting G0 adults/No. tested	Mutations recovered in F1	Allele	DNA sequence	Predicted Mutation
5′ rho,	2/5 (40%)	4	rhoWT	GCCTATGTCCAATGCCACCGGGGTAGTC
codon 17			rhofl6	GCCTATGTCCAATGCCTAACCCTAACCCGGGGTAGTC	p.T17*
			rhofl7	GCCTATGTCCAAT—-CCGGGGGTAGTC	p.A16_T17delinsP
			rhofl10	GCCTATGTCCAATG—CCGGGGTAGTC	p.T17del
			-	GCCTATGTCCA————-AGTC	-
3′ rho,	3/7 (43%)	6	rhoWT	ACCGCCTCCAAGACCGAGGCTTCGTCCGTGTCTTCCAGCTCCGTGTCTCCGGCGTAAA
codon 347			rhofl8	ACCAC-TGTCTGGA-CG—-AAGACCGAGGCTTCGTCCGTGTCTTCCAGCTCCGTGT	p.A338_S339delinsTVWT
			rhofl9	ACCGCCTCCAAGACCGAGGCTTCGTCCGTGT————————AAA	p.S347*
			-	ACCGCCTCCAAGACCGAGGCTTCGTCCGT————————–AAA	-
			-	ACCGCCTCCAAGACCGAGGCTTCGTCCGTGTCTTCCAG——————–	-
			-	ACCG————AGGCTTCGTCCGTGTCTTCCAGCTCCGTGTCTCCGGCGTAAA	-
			-	ACCGCCTCCAAGACCGAGGCTTCGTCCG———————–GCGTAAA	-

Sequence analysis and frequency of germ line transmission of 5′ rho and 3′ rho CRISPR/Cas9-induced alleles aligned to the wild-type rho allele (WT) show that the majority represents deletions and insertions (red text). Blue text represents CRISPR PAM sequence. Underlined DNA represents gRNA target sequence. Alleles characterized in this report are indicated by an allele designation.