Table 4.
Robustness of the method by changing flow rate of the mobile phase and detection wavelength (n = 6).
| Vitamin C |
Gallic acid |
Rutin |
Ellagic acid |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Retention time (RSD)% | Peak area RSD (%) | Retention time (RSD)% | Peak area RSD (%) | Retention time (RSD)% | Peak area RSD (%) | Retention time (RSD)% | Peak area RSD (%) | ||
| Flow rate (mL/min) | 0.8 | 0.88 | 0.44 | 0.28 | 0.38 | 0.78 | 0.36 | 0.58 | 0.88 |
| 1 | 1.02 | 0.53 | 0.87 | 0.58 | 1.25 | 2.11 | 0.40 | 0.53 | |
| 1.2 | 0.45 | 1.58 | 0.66 | 1.55 | 1.23 | 0.52 | 0.87 | 0.74 | |
| Detection wavelength (nm) | 250 | 0.84 | 1.46 | 0.98 | 0.84 | 0.84 | 0.78 | 0.96 | 0.64 |
| 254 | 0.71 | 1.28 | 1.11 | 0.57 | 0.56 | 0.56 | 0.28 | 0.66 | |
| 258 | 1.58 | 0.35 | 0.58 | 0.71 | 0.38 | 0.44 | 1.62 | 1.84 | |