Table 2.
Plasma concentrations of C3 in M. fascicularis monkeys
| Time point | Blood concentration (μg/mL) (n=6) | Mouse levels (at same dose) |
|---|---|---|
| 1.5 h | 2.53 ± 0.21 | 1 |
| 3 h | 3.36 ± 0.45 | 1.5 |
| 24 h | 3.42 ± 0.25 | 2 |
| 1 week | 9.96 ± 0.40 | 5 |
Plasma levels of C3 measured in two M. fascicularis given C3 (7 mg/kg/day) by chronic subcutaneous infusion via osmotic pumps. Using allometric scaling to compare expected blood levels between monkeys and mice receiving the same administered dose, we used the following calculations. The Km for mice was 4.5, based on the 35 g mice used here, and for monkeys, was 9.5 [23]. Thus, the same dose should produce 9.5/4.5=2.1 times higher levels in monkeys compared to mice. The expected concentrations of C3 in monkey plasma based on data from mice given a similar dose (in the last column) and are predicted well by these scaling calculations [23]. Values are mean ± SEM (n=6).