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. Author manuscript; available in PMC: 2019 Sep 6.
Published in final edited form as: Cell. 2018 Aug 16;174(6):1465–1476.e13. doi: 10.1016/j.cell.2018.07.031

Figure 6. Cell-penetrating peptides for several HPV types can deliver fusion proteins into the cytoplasm.

Figure 6.

(A) Schematic diagram of GFP11 fusion proteins containing seven tandem copies of GPF11 (green) linked to amino acids 434 to 455 of HPV16 L2 (blue) and a CPP (red). (B) HaCaT/GFP1–10NES cells were mock-incubated or incubated with GF11–16L2–16CPP or GFP11–16L2-TatCPP for three h. In bottom panels, cells were then treated with trypan blue to quench extracellular fluorescence. Cells were examined by confocal microscopy. (C) Cells were treated as in panel B except there was no trypan blue treatment. GFP11 fusion proteins containing the following CCPs were used: HIV Tat, HPV16 (wild-type, 6A, and 3R mutants), HPV5, HPV18, and HPV31. See Figure S2.