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. Author manuscript; available in PMC: 2018 Sep 8.
Published in final edited form as: Curr Alzheimer Res. 2018;15(10):894–904. doi: 10.2174/1567205015666180507104755

Figure 5. Activation of CREB by cinnamic acid (CA) in mouse BV-2 microglial cells.

Figure 5

(A) Position of CRE in the Socs3 gene promoter. Cells were treated with 300 μM CA for different minutes under serum-free conditions followed by monitoring the level of phospho-CREB (pCREB) by Western blot (B). Graph represents densitometric analysis of pCREB protein levels normalized to total CREB (loading control) (C). Results are mean ± SD of three independent experiments. ap < 0.05 vs control; bp < 0.01 vs control. Cells treated with 300 μM CA for 30 mins under serum-free condition were double-labeled for pCREB & Iba1 (D) and total CREB & Iba1 (E). DAPI was used to stain nuclei. Cells were treated with 300uM of CA under serum-free condition for 15, 30, 60, and 90 min followed by monitoring the DNA-binding activity of CREB by EMSA (F). Results represent three independent experiments. Upper, middle and bottom arrows indicate CREB DNA-binding, non-specific (NS) DNA-binding and unbound probe, respectively.