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. Author manuscript; available in PMC: 2018 Oct 15.
Published in final edited form as: Int J Cancer. 2018 Aug 9;143(8):1963–1977. doi: 10.1002/ijc.31586

Figure 5. Dose-dependent production and secretion of Fn by HNNFbs following stimulation with sCEA.

Figure 5.

A. Activation of HNNFbs by either sCEA (200 ng/mL) or TGF-β (5pg/mL) specifically upregulate Fn expression in a dose-dependent manner. B. Addition of sCEA induces a dose-dependent upregulation of Fn expression in fibroblasts. Immuno-blot analysis of total Fn expression by HNNFbs stimulated with increasing concentrations of sCEA for 24 hours. C. Increased production in total Fn as measured by ELISA, of HNNFbs cultures stimulated by escalating concentrations of sCEA for 24 hours. D. Rise in total Fn secreted by sCEA-activated HNNFb cultures as a function of time. E. AKT-mTOR C1 inhibitors palomid-529 or pentoxifylline reduce the production of Fn by sCEA-activated HNNFbs.