Table 4.

Primer Pairs Used in This Study

Primer	Sequence (5′–3′)	GC%	3′ΔG (kcal/mol)	Amplicon Size (bp)	Ta (°C)
AC007182.6 F	GTAAGTCAGCATGTAGCAC	47.7	−7.5	86	50
AC007182.6 R	GCTCCATCAATAACATCTTCAT	36.4	−5.7
AC009948.5.007 F	ATCAAGTTACAGAGCAGAG	4.1	−64	83	50
AC009948.5.007 R	AACATTACCGAGGACAAC	44.4	−6.5
AL450992.2.001 F	TTAGACTCTCCTTGACCAT	42.1	−6.6	113	52
AL450992.2.001 R	TTCTCCTTCTGTGCTTTC	44.4	−5.7
AL928768.3 F	ACAGGGAGGAAGTGTGGAG	57.9	−6.9	93	56
AL928768.3 R	GTGAGTAAGGGCGGGTC	64.7	−7.4
IL21R-AS1 F	CTCCGACCACTCATTCAG	55.6	−6.1	119	56
IL21R-AS1 R	CTTATCACCTTGCCGTCTG	52.6	−6.6
RP11.126K1.6 F	AACAGTCGCCTTCCAACAC	52.6	−6.5	118	62
RP11.126K1.6 R	TCCATCACTCCTACCCATCATT	45.5	−5.7
RP11-98D18.3 F	AGGCTCAGTCACCTTTCC	55.6	−6.2	81	54
RP11-98D18.3 R	CCTTCTCTGTGACCGTCGA	57.9	−7.4
RP11-290L1.3 F	GCGAGTGCGGCTCGTGATCTC	66.7	−5.8	168	64
RP11-290L1.3 R	CCGGTCAAGCTCAAGGAACTGC	59.1	−7.5
RORC F	TGCCAGAATGACCAGATTGTGCTT	45.8	−7.1	132	60
RORC R	GAACAGCTCCATGCCACCGTA	57.1	−7.1
UBC F	GGATTTGGGTCGCAGTTCTTG	52.4	−6.1	135	58
UBC R	TGCCTTGACATTCTCGATGG	50	−6.5
YWHAZ F	ACTTTTGGTACATTGTGGCTTC	40.9	−6.9	94	62
YWHAZ R	CCGCCAGGACAAACCAGTA	57.9	−5.8

F and R, forward and reverse primers, respectively; 3′ΔG stands for Gibbs energy for binding of the 3′ part of the primer with the template. Ta, annealing temperature of PCR reactions.