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. 2018 Sep 7;8:13442. doi: 10.1038/s41598-018-31713-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence analysis of liver sections. Mice were sacrificed 3 days after vector injection. (A) HAdV17GFP injection induced weak GFP signals in liver sections of C57BL/6 wild type (WT) mice (left), comparing to HAdV5GFP injection (right). (B) HAdV17GFP injection induced high GFP signals in liver sections of CD46 transgenic mice (left), but no detectable signal after PBS injection (right). (C) There are no obvious colocalization of GFP and CD31 in liver sections of WT mice after HAdV5GFP injection. (D) GFP signals were highly colocalized with CD31 in liver sections of CD46 transgenic mice injected with HAdV17GFP. (E) High magnification of GFP signals with CD31 in liver sections of WT mice after HAdV5GFP injection. Scale bars: A-B, 50 µm; C-D, 20 µm; E, 25 µm.