Figure 2.

Gene editing in NUP^Cas9 cells. NUP^Cas9 cells were transduced with constructs encoding for a puromycin resistance gene, RFP and a gRNA and selected with puromycin. (A) Histograms show RFP expression in control NUP-MDSC^Cas9 cells (black) or NUP-MDSC^Cas9 cells transduced with a construct encoding for non-targeting or Itgam-specific gRNA (gray). (B) Histograms show CD11b expression in control NUP-MDSC^Cas9 (black) or NUP-MDSC^Cas9 cells transduced with a construct encoding for non-targeting or Itgam-specific gRNA’s (gray).