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. 2018 Sep 7;8:13429. doi: 10.1038/s41598-018-31759-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Progressive reduction in axonal transport of mitochondria during network maturation. (A) Kymograph analyses at early, mid and late stages of network maturation show a progressive decrease in mitochondria motility. Number in brackets indicates DIV. Anterograde and retrograde movements, pauses and stationary mitochondria are shown. (B) Decreased segmental velocity (Antero, F_3,225 = 3.1, p < 0.05; Retro, F_3,225 = 10.0, p < 0.0001; n = 57) and (C) decreased number of motile mitochondria (Antero, F_3,225 = 15.9, p < 0.0001; Retro, F_3,225 = 18.9, p < 0.0001). (D) The total number of mitochondria remains constant over time. (E) Increased percentage of stationary mitochondria (F_3,225 = 98.9, p < 0.0001). (F) Decreased velocity and number of mitochondria lead to a global decrease in linear flow rate (F_3,225 = 25.7, p < 0.0001) until DIV 14 (DIV 14 vs DIV 21, n.s) but (G) no directional preference could be observed. All bar graphs recapitulate data obtained at early (DIV 4) and late (DIV 21) stages of neuronal culture. ***p < 0.01, ***p < 0.0001.