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. 2018 Sep 7;8:13404. doi: 10.1038/s41598-018-31492-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of the wheat-derived arabinoxylan (AX) on colonic paracellular permeability in biopsies mounted in Ussing chambers. Stimulation with Compound (C) 48/80 (10 ng/ml) resulted in a significantly higher paracellular permeability compared to vehicle (a,b). Co-stimulation with AX (0.1 mg/ml) showed a significant decrease of C48/80-induced hyperpermeability on paracellular passage in only the healthy controls (b). Stimulation with AX only had no significant effect on neither paracellular nor transcellular permeability compared to vehicle. *p < 0.05, **p < 0.01, ***p < 0.001, ns = non-significant. Two elderly with GI symptoms and 3 healthy controls had to be excluded from the FITC-analysis due to technical problems, hence elderly with GI symptoms; n = 16 and healthy controls; n = 18 (AX only, n = 13).