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. 2018 Sep 7;8:13477. doi: 10.1038/s41598-018-31540-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical detection of infectious disease pathogens in puppies. There is positive immunoreactivity to CAdV-1 in the epithelial cells of the bronchus and submucosal glands in puppy #10 (A). Observe interstitial pneumonia with positive immunolabelling for CAdV-2 (B,C) in puppy #13. There is positive immunoreactivity to antigens of Neospora caninum in the lungs (D), cerebrum (E), and myocardium (F) in puppy #1, with concomitant detection of antigens of CAdV-1 (G) and -2 (H). Immunoperoxidase; Bar, (A,C,D,G and H) 50 µm; (E) 20 µm; (F) 100 µm.