Table 2.
Secondary antibody | Western blot | Immunofluorescence staining | Company |
---|---|---|---|
Alexa fluor® 546 goat anti- rabbit IgG (H + L) | — | 1:500 | Invitrogeni |
Alexa fluor® 546 goat anti- mouse IgG (H + L) | — | 1:500 | Invitrogeni |
Alexa fluor® 488 goat anti- mouse IgG (H + L) | — | 1:500 | Invitrogeni |
ECL™ Anti-mouse IgG, Horseradish Peroxidase linked whole antibody (from sheep) | 1:10000 | — | GE Healthcareii |
Blotting Grade Goat Anti-Rabbit IgG (H + L), Horseradish Peroxidase conjugate | 1:10000 | — | BioRadiii |
Company address: iCarlsbad, CA, USA; iiLittle Chalfont, United Kingdom; iiiHercules, CA, USA.