Table 3.
Gene | Forward primer | Reverse primer |
---|---|---|
Gapdh | 5′-CGAGATCCCTCCAAAATCAA-3′ | 5′-GGTGCTAAGCAGTTGGTGGT-3′ |
Keratin 5 | 5′-CAAGCGTACCACTGCTGAGA-3′ | 5′-TCAGCGATGATGCTATCCAG-3′ |
Keratin 10 | 5′-GCTGACCTGGAGATGCAAAT-3′ | 5′-AGCATCTTTGCGGTTTTGTT-3′ |
Filaggrin | 5′-AAGGTTCACATTTATTGCCAAA-3′ | 5′-GGATTTGCCGAAATTCCTTT-3′ |
Loricrin | 5′-GGAGTTGGAGGTGTTTTCCA-3′ | 5′-ACTGGGGTTGGGAGGTAGTT-3′ |
Tight junction protein 1 | 5′-TGCAAAGAGTGAACCACGAG-3′ | 5′-GGCACTTTTCCGAGATTCTG-3′ |
Claudin 1 | 5′-CCGTTGGCATGAAGTGTATG-3′ | 5′-CCAGTGAAGAGAGCCTGACC-3′ |
Occludin | 5′-GATGAGCTGGAGGAGGACTG-3′ | 5′-GCAGATCCCTTCACTTGCTT-3′ |
Desmocollin 1 | 5′-GTGGTCAGCCTTTCGGTTTA-3′ | 5′-TTGGCAAATCCTGATCCTGT-3′ |
Transglutaminase 1 | 5′-GGTGAACTCCCTGGATGACA-3′ | 5′-AAGGGATGTGTCTGTGTCGT-3′ |
Small proline rich pr. 1 A | 5′-GCCCATTCTGCTCCGTATAC-3′ | 5′-GGTTTTGGGGATGCATGGTT-3′ |
Small proline rich pr. 2 G | 5′-CTCTCCACCACACTGATGCT-3′ | 5′-AATGCTCAGGTGGACAAGGA-3′ |
SPINK5 | 5′-ATGCACCAGGGAGCATAATC-3′ | 5′-TACAGGGGAGTCGTCCATTC-3′ |
Primers were synthesized by Microsynth (Balgach, Switzerland).