Fig. 1.

One stereoisomer of COR is a potential JAZ subtype-selective agonist. a Chemical structures of jasmonate derivatives and coronatine diastereomers. b, c Schematic diagram of ligand-induced protein-protein interactions (PPIs) between COI1-JAZ co-receptors; b naturally occurring ligands (i.e., coronatine) can interact with all co-receptors, whereas c the stereochemical isomer (used in this study) can interact with only some co-receptors. d, e Pull down assay of purified GST-COI1 (5 nM) with recombinant proteins expressed in E. coli, including d MBP-JAZ1 (full length, approximately 40 nM), and e MBP-JAZ3 (full length, approximately 40 nM), in the presence of COR derivatives (100 nM). GST-COI1 bound to MBP-JAZ proteins was pulled down with amylose resin and analyzed by immunoblotting. Goat HRP-conjugated anti-GST antibody was used to detect GST-COI1 (black triangles). Rat anti-MBP antibody and goat HRP-conjugated rat-IgG antibody were used to visualize MBP-JAZ protein levels as the input materials (white triangles)