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. 2018 Sep 7;8:13417. doi: 10.1038/s41598-018-31735-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Alignment of the hinge 1 region and adjacent regions of NR3 in C. subsalsa with NR2 in C. subsalsa, NR1 of Heterosigma akashiwo, NRs of four other representative algal species, Thalassiosira pseudonana, Phaeodactylum tricornutum, Dunaliella salina, and Chlamydomonas reinhardtii, as well as NRs in higher plants, Glycine max, Nicotina tabacum, and Zea mays. The single underlined sequences indicate the adjacent domains, showing the absence of the cytb-5 binding domain in NR3 (indicated by grey arrow). The double underlined sequences indicate the motif containing the serine phosphorylation site for 14-3-3 protein binding in NR3 and NRs in higher plants (indicated by black arrow). The identical residues in all sequences are marked by *; the highly and weakly conserved residues are marked by : and ., respectively.