Figure 3.

Molecular phylogenetic analysis of NR amino acid sequences in plants and algae. The tree was generated using the Maximum Likelihood method based on the Le Gascuel 2008 model⁷⁶. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using a JTT model, and then selecting the topology with superior log likelihood value. A discrete Gamma distribution was used to model evolutionary rate differences among sites (+G) and allowed for some sites to be evolutionarily invariable (+I). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. All positions containing gaps and missing data were eliminated. There were a total of 568 positions in the final dataset. Evolutionary analyses were conducted in MEGA7⁷². The tree was rooted by fungal NRs from Wickerhamomyces anomalus, Hebeloma cylindrosporum, and Metarhizium anisopliae. Bootstrap values less than 50 are not shown.