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. 2018 Sep 7;9:3643. doi: 10.1038/s41467-018-06072-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — FRAP of bulk coacervate phase. Bulk coacervate phase (CM-Dex:PLys (4:1 final molar ratio) containing either (i) 0.36 μM FAM-substrate or (ii) 0.36 μM TAM-HH-min. a Output frames from confocal imaging (63×) are shown at t = −0.5 s before bleaching, directly after bleaching (magenta circle, t = 0 s) and t = 13 s after bleaching. The fluorescence intensity was normalised against a reference (green circle) and fit to standard equations. Scale bars are 5 μM. b Plots of normalised FRAP data for HH-min (ii) and FAM-substrate (ii) show the standard deviation (grey, N = 10) and fit (blue) from the same bleach spot radius. c Diffusion coefficients and viscosities obtained from b. Mean and standard deviations are from at least two different samples with analysis from ≥14 bleach spots for each experiment