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. 2018 Sep 7;9:3643. doi: 10.1038/s41467-018-06072-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — RNA catalysis in coacervate microdroplets. a (i) Wide-field optical microscopy images of CM-Dex:PLys (4:1 final molar ratio) coacervate microdroplets prepared in cleavage buffer (1 μM of HH-min and 0.5 μM FRET substrate). Fluorescence microscopy images at t = 0 min (ii) and t = 900 min (iii) show an increase in FAM fluorescence (see inset). Scale bars are 20 μM. b Background corrected and volume/endpoint normalised fluorescence intensity of droplets. Standard deviation of kinetics from 12 micro-droplets (grey) with the mean biexponential fit (blue) and residuals (inset)