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. 2018 Sep 4;11:5419–5428. doi: 10.2147/OTT.S164575

Figure 5.

Figure 5

miR-628 suppressed the mRNA and protein expression of SOS1.

Notes: The breast CSCs of MDA-MB-231 (A) and MCF-7 (B) cells were transfected with the miR-628 mimic and miR-628 inhibitor, and SOS1 mRNA level was determined by qRT-PCR. (C) The breast CSCs of MDA-MB-231 and MCF-7 cells were transfected with miR-628 mimic and miR-628 inhibitor, and SOS1 protein level was analyzed by Western blotting. The protein expression levels were statistically analyzed by quantitating the intensity of the protein bands relative to that of the internal loading control (α-tubulin). Values indicate mean ± SD; *P<0.05.

Abbreviations: CSCs, cancer stem cells; NC, negative control; SOS1, SOS Ras/Rac guanine nucleotide exchange factor; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.