Figure 6.

βE₂ prevents ROS production in H₂O₂-treated ARPE-19 cells. The cells were treated with 10 µM βE₂ for 2 h before exposure to 40 µM H₂O₂ for 24 h.

Notes: (A) Intracellular ROS levels were measured using DCF fluorescence. (B) Statistical analysis of intracellular ROS levels. The data are shown as the mean ± SEM; n=3. ^*P<0.05 vs. control; ^#P<0.05 vs. H₂O₂ treatment.

Abbreviations: βE₂, 17β-estradiol; FITC-A,.