Figure 6.

The Cdc42-Par6 signaling pathway is involved in DKK1-mediated suppression of host cell migration during Cryptosporidium parvum infection. IEC4.1 cells were incubated with the supernatants from infected IEC4.1 cultures for 24 hours, in the presence or absence of the neutralizing anti-Dkk1. Wound healing was applied to the cell cultured as the cell migration assay, followed by immunostaining with anti-Par6 or anti-Cdc42-GTP (Cdc42-active), respectively. Representative images showing the staining of Par6 (A) and Cdc42-GTP (B) in cells along the migrating edge (indicated by arrowheads) after wounding are shown. Data represent means ± SEs from 3 independent experiments. Bar = 20 µm.