Figure 10. NF-κB mediates albumin-induced SGLT2 mRNA expression in human cultured podocytes.

SGLT2 mRNA expression evaluated by real-time qPCR analysis in podocytes exposed to medium alone (control) or albumin (10 mg/ml) in the presence or absence of SN50 (10 μM) for 3 hours. Hypoxanthine phosphoribosyltransferase 1 (HRPT1) was used as endogenous control. SGLT2 levels were normalized to HPRT1 levels and reported as fold change relative to control. Data are the mean ± SEM (n = 4 samples) and were analyzed by ANOVA with Tukey’s post hoc test.