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. 2018 Aug 9;3(15):e98720. doi: 10.1172/jci.insight.98720

Figure 8. Human cultured podocytes express SGLT2 that is upregulated by albumin.

Figure 8

(A) Representative Western blot and densitometric analysis of SGLT2 protein in renal proximal tubular cells (RPTECs), used as positive control, and podocytes exposed to medium alone (control) or albumin (10 mg/ml, 6 hours). Actin was used as sample loading control. Data are the mean ± SEM (n = 4 samples) and were analyzed by Student’s t test. (B) SGLT2 mRNA expression evaluated by real-time qPCR analysis in podocytes exposed to medium alone (control) or albumin (10 mg/ml) for 3 and 6 hours. Hypoxanthine phosphoribosyltransferase 1 (HRPT1) was used as endogenous control. SGLT2 levels were normalized to HPRT1 levels and reported as fold change (FC) relative to the corresponding control. Data are the mean ± SEM (n = 6 samples) and were analyzed by ANOVA with Tukey’s post hoc test.