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. 2018 Aug 9;3(15):e120159. doi: 10.1172/jci.insight.120159

Figure 4. HDAC11 deficiency protects against deleterious effects of chronic high-fat feeding.

Figure 4

(A) Schematic representation of the experiment. (B) Weight gain (%) was calculated at 12 months; n = 4 WT, n = 5 KO. (C) Glucose tolerance was assessed in mice following 12 months of high-fat feeding; n = 4–5/group. (D and E) Plasma insulin and leptin concentrations were determined by ELISA; n = 4/group. (F) Plasma adiponectin concentrations were determined via immunoblotting and normalized to IgG levels; n = 4/group. (G and H) Body composition (total fat and lean mass) was determined using QMRI; n = 4 WT, n = 5 KO. (I) H&E staining of iBAT, eWAT and liver following 12 months of high-fat feeding. Scale bars: 10 μm. (J–L) Adipocyte area in iBAT and eWAT and lipid accumulation in liver were quantified as described in Methods.; n = 4 WT, n = 5 KO. Values for all graphs represent mean ± SEM; *P < 0.05 vs. WT. Two-way ANOVA with Šidák’s multiple-comparisons test for 4 groups was employed for C; Student’s t test was used for all other graphs.