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. 2018 Aug 9;3(15):e120159. doi: 10.1172/jci.insight.120159

Figure 7. Mapping of HDAC11:BRD2 association domains.

Figure 7

(A) Schematic representation of human HDAC11 and carboxy terminal deletion constructs. (B) Schematic representation of the HDAC11:BRD2 co-IP assay with HDAC11 deletion constructs. (C) Immunoblot assessment of endogenous BRD2 coimmunoprecipitating (co-IPed) with ectopic HDAC11. Total amounts of HDAC11 and BRD2 in protein homogenates were also determined (Input). (D) Schematic representation of human BRD2 and a carboxy terminal deletion construct. BD1, bromodomain 1; BD2, bromodomain 2; ET, extraterminal domain. (E) Schematic representation of the HDAC11:BRD2 co-IP assay with ectopic HDAC11 and ectopic BRD2. (F) Immunoblot assessment of ectopic BRD2 co-immunoprecipitating with ectopic HDAC11. Total amounts of epitope-tagged HDAC11 and BRD2 in protein homogenates were also determined (Input).