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. 2018 Aug 9;3(15):e121739. doi: 10.1172/jci.insight.121739

Figure 5. PDE5 inhibition in the normal heart and stressed lung.

Figure 5

(A) qRT-PCR was performed for various miRs in tissue from normal, nonstressed C57BL/6J mice treated with PDE5-I (Sil) for 5 weeks (n = 5 per group). Cardiac miRs that normally decrease with PO (top row) and increase with PO (bottom row) assessed from left ventricular tissue. Only miRs that were significantly decreased by Sil in Figure 4 were assayed. The dashed line in each graph represents the level to which Sil reduced miR expression in the PO model. (B) Lung tissue from C57BL/6J mice subjected to PO, PO+PDE5-I (Sil), or sham surgeries was analyzed by qRT-PCR for lung miRs (n = 5–6 per group). **P < 0.01, ***P < 0.001, ****P < 0.0001 as compared with control for sham Sil heart analysis. Data are presented as the mean ± SEM. For A, data were analyzed using 2-tailed unpaired t tests. For B, data were analyzed using 1-way ANOVA with Dunnett’s post hoc test, or Kruskal-Wallis test with Dunn’s post hoc test.