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. 2018 Aug 9;3(15):e99292. doi: 10.1172/jci.insight.99292

Figure 3. Dichloroacetate (DCA) treatment increases mitochondrial respiration and improves the metabolic potential of mitochondria in splenocytes from septic mice.

Figure 3

Seahorse XF24 Analyzer and the mitochondrial stress test was used to determine oxygen consumption rate (OCR) in splenocytes from SHAM (n = 4), cecal ligation and puncture (CLP) (n = 6), or CLP+ DCA (n = 6) mice. A shows tracing of average values from SHAM, CLP, and CLP+ DCA groups. (B) OCR data generated using the mitochondrial stress test by Seahorse WAVE 2.4 Software (Agilent) was analyzed using GraphPad Prism 7.04 Software and ANOVA with Fisher’s LSD post-test. (C) The Seahorse WAVE 2.4 Software was used to evaluate the metabolic potential of mitochondria using the extracellular acidification rate (ECAR) and OCR in splenocytes from SHAM (n = 4), CLP (n = 6), and DCA-treated CLP mice (n = 6) as measured by the Seahorse XFe24 Analyzer.